Results 1 to 3 of 3

Thread: Gene Cloning

  1. #1
    Join Date
    Aug 2007
    Rep Power

    Default Gene Cloning

    Gene Cloning
    Gene cloning is a process by which large quantities of a specific, desired gene or section of DNA may be cloned or copied once the desired DNA has been isolated.
    Method of Gene Cloning:
    1) The gene or DNA that is desired is isolated using restriction enzymes.
    2) Both the desired gene and a plasmid are treated with the same restriction enzyme to produce identical sticky ends.
    3. The DNAs from both sources are mixed together and treated with the enzyme DNA ligase to splice them together.
    4. Recombinant DNA, with the plasmid containing the added DNA or gene has been formed.
    5. The recombinant plasmids are added to a culture of bacterial cells. Under the right conditions, some of the bacteria will take in the plasmid from the solution during a process known as transformation.
    6. As the bacterial cells reproduces (by mitosis), the recombinant plasmid is copied. Soon, there will be millions of bacteria containing the recombinant plasmid with its introduced gene.

    7. The introduced gene can begin producing its protein via transcription and translation.  885

  2. #2
    Join Date
    Aug 2007
    Rep Power


    also check out this cool animaion.......

    CLICK HERE  885

  3. #3
    Join Date
    Mar 2009
    Rep Power


    I'm mad.

Thread Information

Users Browsing this Thread

There are currently 1 users browsing this thread. (0 members and 1 guests)

Similar Threads

  1. Doc claims cloning three human babies
    By trimurtulu in forum Articles
    Replies: 0
    Last Post: 03-05-2009, 07:48 AM
  2. Tumor Suppressor Gene: Gene Function 'Lost' In Melanoma And Glioblastoma
    By trimurtulu in forum Latest Research And Medical News
    Replies: 0
    Last Post: 12-25-2008, 07:39 AM
  3. The Facts and Fiction of Cloning
    By PreDator in forum Articles
    Replies: 0
    Last Post: 12-12-2007, 05:24 PM
  4. UN report: Human cloning ban needed
    By drchinx in forum Current Affairs
    Replies: 3
    Last Post: 11-16-2007, 06:15 PM


Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts